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(1998) pCrGFP, pKScGFP Synthetic GFP reporter optimized for nuclear codon bias; ... (2004) pRbcRL(Hsp196) Synthetic luciferase reporter gene, controlled by ... using the Cre/lox-system Heitzer and Zschoernig (2007) Lodha et al., 2008).. Browse Addgene's collection luciferase plasmids assays transcriptional regulation. ... 106460, pCR3.1-CREM2 luc, luciferase reporter, CMV promoter (CRE site .... Componets 1 CRE(1)Luciferase Reporter Vector (20ul of 500 ng/ul) ... cAMP response element (CRE), including Jun N-terminal kinase (JNK), p38, and.. ... Plasmid or Mock Plasmid (A) Hek293T, CHO or Huh-7 cells were transfected with CRE-luciferase reporter constructs and a human TGR5 expression plasmid .... could introduce the plasmid into the terminally differentiated iCell. Cardiomyocytes ... Similarly, luciferase-based CRE and NFAT reporters acted as a surrogate .... by I Manual — Plasmid Information). In addition to the luciferase reporter format, the CRE, AP-1, NF-kB and. NFAT cis-reporting plasmids are available with an hrGFP reporter.. by D Akhmedov · 2016 · Cited by 7 — The ROSA26-CRE-luc mouse line is a useful resource to study ... we obtained a validated CREB-activated luciferase reporter plasmid from .... by M Schwaninger · 1993 · Cited by 89 — Fax: 551-399652. The abbreviations used are: CRE, cAMP response element; CaM ... Plasmid Construction-The plasmids -350GluLuc and. 5xCRET81Luc ... acetyltransferase assay (20) and the luciferase assay were performed as previously .... The key to the CRE/CREB Transient Pack is the CRE/CREB luciferase reporter vector, which is a cAMP/PKA Cell Signaling Pathway-responsive reporter.. by N Pedersen · Cited by 1 — Keywords: cloning, Cre recombinase, plasmid, reporter genes, therapeutic genes. Abbreviations: ... promoter using a variety of reporter genes (e.g. luciferase.. by O Ernst · Cited by 7 — promoter luciferase reporter gene construct, a kind gift from Dr. S. Smale [15], was amplified using ... 2.10 Construction of plasmids - The CRE consensus x4 .... by H Li · 2020 — (F) Construction of PA-Dre in a single plasmid. Schematic ... fold) but lower luciferase reporter activity than Magnets–PA-Cre. (SI Appendix, Fig.. The CRE reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency.. by OG CHEPURNY · 2007 · Cited by 58 — Plasmid DNA for all experiments was obtained from transformed ... Characterization of a novel luciferase reporter incorporating the CRE of .... by MD Conkright · 2003 · Cited by 650 — late CRE-dependent transcription via a phosphoryla- tion-independent ... taining EVX-1 luciferase reporter vector (Conkright et which CREB .... by G Thiel · 2005 · Cited by 38 — (D) The reporter plasmid pTNFα(CRE/AP1)2luc contains two ... the regulation of the glucose-6-phosphatase promoter/luciferase reporter genes .... by LR Watkins · 2021 · Cited by 1 — For CRE and NFAT luciferase reporter assays, cells were incubated ... Plasmids encoding the following GPCRs were obtained from cDNA .... by Z Cheng · 2010 · Cited by 133 — Keywords: Luciferase, Reporter assays, GPCR, profile of receptor/G protein coupling. INTRODUCTION ... cluding cAMP response element (CRE), nuclear factor of ... reporter vector and receptor (D1 dopamine receptor and m3.. This strain serves as a Cre reporter strain. When used ... strain, successful Cre-mediated excision is indicated by luciferase expression in Cre-expressing tissues.. 2.1 Promoter Studies with Dual-Luciferase Reporter Assays. 12. 2.2 Signal ... 2.3 Vector map of a co-reporter plasmid for dual-reporter assays. In this vector, the reporter ... downstream genes. CRE: Cyclic-AMP response element; SRE: Serum.. Cignal Reporter Assays are based on dual-luciferase technology, and generate exceptionally ... control, C a constitutively expressing GFP construct containing Monster Green® ... elevate intracellular cAMP, compared to the CRE reporter that.. PSF-CAG-RENILLA - CAG LUCIFERASE REPORTER PLASMID (snapfast vector); plasmid vector for molecular cloning; < B> Cloning in a gene: < /B> This .... Luciferase Reporters in Circadian Research · GFP tagging (Green Fluorescent Protein fusion) · What is .... AAV-CMV-lacZ. The following viruses are available in AAV2 & AAV5: AAV-CMV-Cre-GFP; AAV .... Spike(SARS Plasmid Lenti-luciferase-P2A-Neo from Dr. Christopher Vakoc's lab ... reporters Protein tags HA G.O.I. FL AG + Puromycin CRE recombinase G.O.I. .... The pGL2 Luciferase Reporter Vectors(a) are designed for the quantitative analysis of factors ... Expression of luciferase activity in cells transfected with this plasmid depends on insertion and ... pGL4.29[luc2P/CRE/Hygro] Vector. 20 g. E8471.. However, placing the reporters on different plasmids separated the two ... firefly luciferase were isolated, they were tested for the extent of CRE-regulation.. by Y Chen · Cited by 10 — Both cell lines were observed a cAMP-inducible luciferase expression after transfection of the reporter plasmid CRE-luc. Stimulated by 5-.. by Q Shan · 2010 · Cited by 11 — Luciferase assay. Cells were transfected with the CRE-luciferase reporters and EF1-LacZ plasmids. The EF1-LacZ plasmid contains the β- .... The pseudotype system was then extended by using a three-plasmid system ... The third gene ( luc ) encodes the reporter luciferase which was included to study ... reporters Protein tags HA G.O.I. FL AG + Puromycin CRE recombinase G.O.I. .... by Y Chen · 2015 · Cited by 10 — Both cell lines were observed a cAMP-inducible luciferase expression after transfection of the reporter plasmid CRE-luc. Stimulated by 5-HT for .... cre/+ FIGURE 4|Functional and RNA sequencing analysis of PRMT5-deleted Tregs. ... Human CD4+ T cells were retrovirally transfected with the empty vector, FOXP3 ... of its target gene promoters by IL-2 promoter luciferase assay (Figure 5E).. Luciferase assay reagent (Promega, Madison, WI, USA). ... or pSVL-TSHR mutant constructs and a cAMP-response element (CRE)-luciferase reporter plasmid.. ... cells [ 15 ] , mutation of the COX - 2 CRE severely represses COX - 2 induction in RAW ... CREB over - expression blocks luciferase induction from the COX - 2 ... which blocks LPS induction of an NFKB - luciferase reporter , does not block LPS ... Cotransfection with a plasmid expressing a DN - ECSIT protein significantly .... by SJ Kaczmarczyk · 2003 · Cited by 15 — The use of cre recombinase to activate or inactivate gene expression ... Transfections with luciferase reporter plasmids were performed 2 h later .... In this approach, the adenoviral vector-expressing Cre driven by the PSA promoter-enhancer activates expression of the secondary CMV-loxP-stop-luciferase .... All PCR reagents and kits for plasmid isolation were obtained from Qiagen ... μl of the lysates using Dual Luciferase® Reporter Assay System (Promega, USA). ... of human GSTP1 promoter) - 5'-CTTAGG GAA TTT CCCCCC GC-3', CRE (CRE .... 468 products — ... next to the gene promoter of interest within a vector and transfected to a cell line. ... CRE Luciferase Reporter-HEK293 Cell Line ... The CRE Luciferase Reporter cell line is a stably transfected HEK 293 cell line which expresses.. When this Luciferase reporter adenovirus and an uncharacterized gene are cotransfected into mammalian cells, the activation of endogenous protein kinases .... The CRE reporter is premixed with constitutively-expressing Renilla (sea pansy) luciferase vector that serves as an internal control for transfection efficiency.. ID Plasmid Gene/Insert PI ; 9052: pBABE puro K-Ras V12: KRAS (Homo ... Feng Zhang's lab contains the inserts sgRNA, Renilla luciferase, Cre recombinase, ... L861Q) Supported Keywords: Assay IDs Entrez Gene IDs Gene Symbols Gene .. Retroviral vectors were used to deliver luciferase reporter genes because these ... The CREB responsive vector pCRE-IL2-Luc contains CRE sequences that .... The SRE reporter assay follows the same principle than the CRE reporter assay. ... Therefore, HEK-293 cells were transfected with a SRE-Luc reporter plasmid ... Luciferase assay reagent was added and related luminescence was measured.. Jun 28, 2021 — The novel cyclic adenosine monophosphate (cAMP)-responsive luciferase reporter exhibits optimal performance in high-throughput screens of .... by TER ASSA — interest into an expression vector that is then transferred into ... Luciferase reporter technology gives the researcher unparalleled sensitivity, dynamic range, versatility and ease of ... stable cell line with a CRE/promoter-luciferase introduced.. The pGL4.29[luc2P/CRE/Hygro] Vector contains a cAMP response element (CRE) that drives the transcription of the luciferase reporter gene luc2P (Photinus .... 1999), Rosa-26-based reporter (Rosa-GFP) mice (GFP activation after Cre ... (B) Conditional expression of mRFP and luciferase mediated by the Cre/loxP .... G.O.I. P2A Luciferase Luminescent reporters Protein tags HA G.O.I. FL AG + Puromycin CRE recombinase G.O.I. expression P2A self-cleavage G.O.I. ... (B) MCF-7 or (C) B16-F10 cells were transduced with a lentiviral vector containing the .... Cyclic AMP Response Element (CRE/CREB) CRE-Luc, CREB-Luc Response Element ... A luciferase reporter gene can be engineered into organisms or cells, allowing ... Vector Type: pLT-Luc or pLT-Luc-Marker (see the vector map above).. by I Manual — Plasmid Information). In addition to the luciferase reporter format, the CRE, AP-1, NF-kB and. NFAT cis-reporting plasmids are available with an hrGFP reporter.. by S Li · 2018 · Cited by 14 — In the conditional Cre/loxp reporter system, the first reporter gene is flanked by two loxp ... The GFP from the jellyfish, Aequorea victoria, discovered in 1962 by ... Szyska et al69 generated dual-luciferase reporter mouse model .... Luciferase reporter assay systems are successfully used in the drug discovery process against ... in the same vector as CRE-firefly luciferase. However, no such.. by J Xavier-Neto · 2004 · Cited by 6 — This suggests that CRE participates in the regulation of the ACE gene in endothelial cells. ... Cell lysis buffers and the luciferase assay system were from Promega ... with 3.25 µg of luciferase reporter plasmids and 0.48 µg of a control plasmid .... ... transduction and expression of the target genes as well as antibiotics resistant genes or reporters. ... Cre (PGK) Firefly Luciferase (CMV) Lentivirus (2x25uL).. May 12, 2010 — The miRNA control assays are unique to each assay chemistry . ef1a ... Promoters for Gene Expression Lentiviral vector with internal EF1a promoter, ... AAV with EF1a promoter driven Cre . ... The three promoters were put into the same expression vector, pAC-luc, driving expression of the luciferase cDNA.. Sep 3, 2010 — Binding of transcription factors, such as the cAMP response element binding protein (CREB), to the CRE sequence allows Metridia luciferase ( .... The GLuc-ON™ CREB TRE lentiviral clone contains tandem repeats of CRE upstream of ... LF061, Secrete-Pair Gaussia Luciferase Assay Kit (100 rxns), Detects ... HIV-based lentiviral packaging plasmids, eGFP control clone and EndoFectin .... tronic Q vector construct (QC-FLAG-ATF5-eGFP) for retrovirus production. The CRE-luciferase reporter plasmid was constructed by annealing synthetic oligo .... by M Safran · 2003 · Cited by 132 — using a viral vector or by mating to one of an expanding number of transgenic Cre mouse strains. Bioluminescent imaging based on luciferase expression is a .... Article Snippet: Expression of a CRE-luciferase reporter gene was induced by cotransfection with the pFC-PKA plasmid expressing the catalytic subunit of .... The non-inducible luciferase vector contains the firefly luciferase gene under the control of a minimal promoter, but without any additional response elements. The .... Luciferase reporter assays are used widely to investigate cellular signaling ... expression in mammalian cells, and the pGL4 plasmid backbone is engineered.. The luciferase gene is subcloned from a pGL3-basic vector and inserted into a p4xCRE-blax vector and ... Luciferase Assay of CHO-K1/CRE/Luc Reporter Cells:.. Dec 18, 2020 — COS-2 cells were transfected with plasmids encoding either the firefly luciferase or GFP reporter of Cre recombinase activity described above.. The pCRE Tluc16-DD Vector is a transcriptional reporter vector designed to monitor the activation of cAMP-binding protein (CREB) and cAMP-mediated signal .... by LC Mattheakis · 1999 · Cited by 12 — assay, we cotransfected Jurkat cells with plasmids. NFAT–Cre and pLM255. A control plasmid, NFAT–Luc, that expresses luciferase directly .... by BK Koo · 2012 · Cited by 696 — Gene-expression profiling of sorted LGR5–GFP cells allowed us to define a ... specific Cre lines (Cyp1a1-cre or villin (Vil)-creERT2), which allow ... surface levels of frizzled receptors. a, Luciferase assay of TCF4–b-catenin.. CREB Responsive Luciferase Reporter HEK293 Stable Cell Line is derived from ... Cyclic AMP response element (CRE)-binding protein (CREB) is a ... line by co-transfecting CREB luciferase reporter vector and hygromycin expression vector.. by M Ohtsuka · Cited by 4 — Cotransfection with pCRTEIL and Cre-expression plasmid (pCAG/NCre) caused ... Luc assay was performed using a kit (Dual-Luciferase Reporter Assay .... MRTX849 Plasmid pHAGE-KRAS-G12V from Dr. Kenneth Scott's lab contains ... L861Q) Supported Keywords: Assay IDs Entrez Gene IDs Gene Symbols Gene . ... Zhang's lab contains the inserts sgRNA, Renilla luciferase, Cre recombinase, .... Results 1 - 12 of 42 — ... activation of intracellular signal transduction pathways. Cotransfection of a cis-reporter plasmid with an expression plasmid shows direct or .... Products 1 - 25 of 44 — The CRE motif (as Transcription response element, TRE) provides a system for study ... report (Luciferase, Renilla Luc) or a fluorescent report (GFP, RFP) or a ... The luciferase signal can be measured via Luciferase assay.. To perform a luciferase reporter assay, you will need a DNA plasmid to express the protein that you hypothesize could affect transcription. You will also need to ... 6e846d8fd7